Endocrine Abstracts

Introduction: Thyrotropin (TSH) and the gonadotropins (FSH, LH, hCG) are a family of heterodimeric glycoprotein hormones composed of two noncovalently linked subunits, α and β. hTSH, heterodimer was converted to a biologically active single-peptide chain, by fusing the common α subunit to the carboxyl-terminal end of hTSHβ subunit in the absence (hTSHβα) or presence of a ~30 aminoacid carboxyl-terminal peptide fr...

Thyrotropin (TSH) and the gonadotropins (FSH, LH, hCG) are a family of heterodimeric glycoprotein hormones composed of two noncovalently linked subunits, α and β. The hTSH heterodimer was converted to a biologically active single-peptide chain (hTSHβCTPα), by fusing the common α subunit to the carboxyl terminal end of hTSHβ subunits in the presence of a ~30 aminoacid peptide from hCGβ (CTP) as a linke...

Objectives: The main goal of the present study is to investigate the role of N-linked oligosaccharides on the structure and function of human thyrotropin (hTSH). A second aim of the present study is to design new analogs of hTSH.Methods: Overlapping PCR technique was used to convert hTSH heterodimer to a biologically active single chain by fusing the α subunit to the carboxyl terminal end of hTSHβ subunit in the absence (hTSHβα) or pr...